Azadirachta indica were used in this present study to evaluate the antibacterial activity. Azadirachta indica were considered as the most effective and showed the maximum zones of inhibition against bacterial strains when compared to the other extracts due to some compounds are present in bark extracts. Hence it was selected for GC-MS analysis.
In present study the phytochemical constituents such as total phenol, total flavonoid contents and antibacterial activity against four gram negative and two gram positive isolates Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogens, Proteus vulgaris, Lactobacillus, S.
Maximum zone of inhibition was recorded in the presence of free flavanoid fraction of the plant extract against Trichophyton mentagophytes and Microsporum fulvum which was the most susceptible fungus for all the extracts tested. The extract also compared favourably with streptomycin which serves as a positive control.
Copy the following to cite this article: Biosci Biotech Res Asia ;14 3. Copy the following to cite this URL: Medicinal plants, which form the backbone of traditional medicine, in the last few decades, have been the subject for very intense pharmacological studies.
Almost all parts of this plant have been used traditionally for treatment of several ailments due to their multiple biological activities such as antihelmintic Patel et al.
Most importantly, the flower extracts of L. Recently, there is a revival of interest in the use of plants as natural remedy for medication of several health disorders due to the reason that they possess multiple biological activities, compatibility with system biology, potential physiological functions and protective role against several degenerative diseases Suhaj, ; Tadhani et al.
The extraction of antioxidant components from a plant material is a crucial step so as to accomplish further fractionation, isolation, purification and characterisation of biologically active compounds. A variety of extraction techniques such as orbital shaker, stirring, accelerated solvent extraction, microwave assisted extraction and supercritical fluid extraction etc.
All techniques have some advantages and disadvantages over others, but none of these is claimed to be perfect in all aspects. In view of the above-mentioned reports, this study was planned to explore the availability of potent antimicrobial agents of L.
Sterilized distilled water and dimethyl sulfoxide DMSO were used as negative control. Processing of Plant Materials The leaves and flowers of L. The dried samples were ground separately into fine powder. About g each of the powdered specimen was soaked in the solvents of acetone, chloroform, petroleum ether, diethyl ether by using soxhlet extraction method for the extraction of pure solvent until the colour of solvent became white.
MHA medium was poured into a well-labelled Petri dish and allowed to set. Holes or wells were then bored into the set inoculated MHA using sterile cork borer. The plates were left on the bench for about one hour to allow proper diffusion of the extract into the MHA. This procedure was repeated for each bacterial isolate.
After 24 hours the plates were observed for clear zone of inhibition, which indicate the relative susceptibility of the bacteria to the extract. The diameter of the zones of inhibition was measured and recorded in mm.
Antifungal Sensitivity Test by Biomass Reduction Method The crude extract was screened for antifungal activity using biomass reduction method. After 48 hrs, the broth culture was filtered through pre-weighted Whatman filter paper. Filter paper was dried inside an oven at 50oC for overnight. After drying, the biomass was further weighted and percentage reduction in biomass was calculated using the following formula Where, w0 is Initial wt.
For each extract of leaf and flower of L. In each case, the spots were visualized by exposure of plates to iodine vapour.
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For UV-Vis spectrophotometer analysis, the extracts were centrifuged at rpm for 10 min and filtered through Whatmann No.
The sample is diluted to 1: The extracts were scanned in the wavelength ranging from nm using Perkin Elmer Spectrophotometer and the characteristic peaks were detected. The wavelength of light absorbed is characteristic of the chemical bond as can be seen in the annotated spectrum.
By interpreting the infrared absorption spectrum, the chemical bonds in a molecule can be determined. The powdered sample of each plant specimen was loaded in FTIR spectroscope Shimadzu, IR Affinity, Japanwith a scan range from to cm-1 with a resolution of 4 cm Results Antibacterial Activity of L.
Camara Leaf Extract Figure 1 shows that L. Extracts were prepared in petroleum ether, diethyl ether, chloroform and acetone.
However only chloroform extract was found to be the most effective against all the bacteria except Pseudomonas and E. Antibacterial activity of L. Camara Flower Extract L. The extracts were prepared in petroleum ether, di ethyl ether, chloroform and acetone.
However only chloroform extract was found to be the most effective against all the bacteria except E. The extracts were prepared in petroleum ether, diethyl ether, chloroform and acetone.Chanca piedra is a small, erect, annual herb that grows 30–40 cm in height.
It is indigenous to the rainforests of the Amazon and other tropical areas throughout the . aims of this study were to evaluate in vitro phytochemical, antimicrobial, cytotoxic and antioxidant activities and to screen for phytochemical constituents of Blepharis genus.
[Family Acanthaceae] Methonolic crude extract. Phytochemical Constituents and Antimicrobial and Grain Protectant Activities of Clove Basil (Ocimum gratissimum L.) Phytochemical Constituents and Antimicrobial and Grain protectant Activities of agar streak dilution antimicrobial activity test of Ocimum gratissimum extracts.
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This study investigated the phytochemical constituents and antimicrobial activity of hexane, ethyl acetate, dichloromethane and methanol leave extracts of Amaranthus hybridus, Amaranthus spinosus and Amaranthus caudatus.
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